semi dry blotter (Hoefer)

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Hoefer semi dry blotter
Semi Dry Blotter, supplied by Hoefer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/semi+dry+blotter/dry+semi+transfer/pm41006428-73-42-44
Average 86 stars, based on 1 article reviews

semi dry blotter - by Bioz Stars, 2026-09

86/100 stars

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Electrophoresis:

Article Title: Role of the ERK Pathway in the Activation of Store-mediated Calcium Entry in Human Platelets
Article Snippet: .. One-dimensional SDS electrophoresis was performed with 10% polyacrylamide minigels and separated proteins were electrophoretically transferred, for 2 h at 0.8 mA/cm2, in a semi-dry blotter (Hoefer Scientific, Newcastle, Staffs., UK) onto nitrocellulose for subsequent probing. ..

Staining:

Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy
Article Snippet: For further testing of MGL interaction, each electrophoretic separation in the gel contained two control lines, namely 1 μg of GalNAc-BSA glycoconjugate (Abcam, Cambridge, UK) as a positive control and 1 μg of bovine serum albumin (BSA) (SERVA Electrophoresis GmbH, Heidelberg, Germany) as a negative control. .. After separation, the gels were divided and subjected to: Coomassie Brilliant Blue (CBB) staining (0.5% CBB in 40% methanol and 10% acetic acid)—for further analysis using mass spectrometry; silver staining ; or transfer of separated amniotic proteins to a nitrocellulose membrane (NC) using semi-dry blotter (Hoefer Inc., Holliston, MA, USA; 120 mA, 90 min). .. The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca 2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking.

Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy.
Article Snippet: For further testing of MGL interaction, each electrophoretic separation in the gel contained two control lines, namely 1 μg of GalNAc-BSA glycoconjugate (Abcam, Cambridge, UK) as a positive control and 1 μg of bovine serum albumin (BSA) (SERVA Electrophoresis GmbH, Heidelberg, Germany) as a negative control. .. After separation, the gels were divided and subjected to: Coomassie Brilliant Blue (CBB) staining (0.5% CBB in 40% methanol and 10% acetic acid)—for further analysis using mass spectrometry; silver staining59; or transfer of separated amniotic proteins to a nitrocellulose membrane (NC) using semi-dry blotter (Hoefer Inc., Holliston, MA, USA; 120 mA, 90 min). .. The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking.

Mass Spectrometry:

Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy.
Article Snippet: For further testing of MGL interaction, each electrophoretic separation in the gel contained two control lines, namely 1 μg of GalNAc-BSA glycoconjugate (Abcam, Cambridge, UK) as a positive control and 1 μg of bovine serum albumin (BSA) (SERVA Electrophoresis GmbH, Heidelberg, Germany) as a negative control. .. After separation, the gels were divided and subjected to: Coomassie Brilliant Blue (CBB) staining (0.5% CBB in 40% methanol and 10% acetic acid)—for further analysis using mass spectrometry; silver staining59; or transfer of separated amniotic proteins to a nitrocellulose membrane (NC) using semi-dry blotter (Hoefer Inc., Holliston, MA, USA; 120 mA, 90 min). .. The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking.

Silver Staining:

Membrane:

Article Title: Amniotic fluid glycoproteins as potential ligands for macrophage galactose-type C-type lectin and their possible implications for immunoregulation during pregnancy.
Article Snippet: For further testing of MGL interaction, each electrophoretic separation in the gel contained two control lines, namely 1 μg of GalNAc-BSA glycoconjugate (Abcam, Cambridge, UK) as a positive control and 1 μg of bovine serum albumin (BSA) (SERVA Electrophoresis GmbH, Heidelberg, Germany) as a negative control. .. After separation, the gels were divided and subjected to: Coomassie Brilliant Blue (CBB) staining (0.5% CBB in 40% methanol and 10% acetic acid)—for further analysis using mass spectrometry; silver staining59; or transfer of separated amniotic proteins to a nitrocellulose membrane (NC) using semi-dry blotter (Hoefer Inc., Holliston, MA, USA; 120 mA, 90 min). .. The membranes were blocked overnight using 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl (TBS) with 1% Tween-20 solution and then incubated with: (1) recombinant human MGL (CLEC10a) protein (Elabscience, Houston, Texas, USA) diluted 1:500 in 15 mM Tris–HCl buffer, pH 7.4, with 0.15 M NaCl; 0.1% Tween-20 (TBST) containing 2 mM Ca2+ or additionally 25 mM GalNAc; (2) biotinylated lectin from Vicia villosa (VVL, Vector Labs, Burlingame, CA, USA) diluted 1:200 in TBST; and (3) biotinylated lectin from Wisteria floribunda (WFL, Vector Labs, Burlingame, CA, USA) diluted 1:500 in TBST, for 1 h at room temperature (RT) with constant gentle shaking.

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